| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.198772 |
| Chromosome: | chromosome 1 |
| Location: | 6508380 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g046324 | (1 of 1) PF14906 - Domain of unknown function (DUF4495) (DUF4495) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATCCCCTCCCCTCCCCGCCCCACCCCT |
| Internal bar code: | GATTTCGGCCTTGCGTGCTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 20.4545 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 245 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTCCTCCTCCCCATCG |
| Suggested primer 2: | GTGTGTAGCAGTCTGGGGGT |