| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198774 |
| Chromosome: | chromosome 3 |
| Location: | 3663660 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169000 | (1 of 26) IPR011990 - Tetratricopeptide-like helical domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACTCTCTCCTTGCGCTTATCCAACAAGC |
| Internal bar code: | GACGTTTTATAGTACCTAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 716 |
| LEAP-Seq percent confirming: | 97.1693 |
| LEAP-Seq n confirming: | 1991 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAATGCAGTCAGAGCCGTG |
| Suggested primer 2: | GAGCGCAAGGAATACAAAGC |