Insertion junction: LMJ.RY0402.198777_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.198777
Chromosome:chromosome 3
Location:9093734
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g211073 (1 of 2) K14497 - protein phosphatase 2C (PP2C) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAATGTCTGTAGGGGTCGGAACAGCGAAAA
Internal bar code:ATGCCTGTCGGGCCTGCTACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:606
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1012
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCTGTCGACTGCTTGTCACC
Suggested primer 2:CCCTGCGGTATACACGTTCT