| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198799 |
| Chromosome: | chromosome 2 |
| Location: | 6395395 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAACAATTGAGACCGCTGCTCAAGTGCC |
| Internal bar code: | CACGGGGCATACGTGGTCCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 98.6177 |
| LEAP-Seq n confirming: | 4138 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGGTTACGTGTGCAGAT |
| Suggested primer 2: | CCACCTGCAGTTCTGTGAGA |