| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.198816 |
| Chromosome: | chromosome 1 |
| Location: | 856959 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004450 | CPLD42 | (1 of 5) PF07466 - Protein of unknown function (DUF1517) (DUF1517); conserved expressed membrane protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCGGCCCTGTGTTTCCACCCTCCGTCGC |
| Internal bar code: | ATTGAGGGCTTTATGTCGCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 99.5389 |
| LEAP-Seq n confirming: | 1511 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGGCGTAAAAGAAAAAG |
| Suggested primer 2: | CTCTCGCCGATCCAACTAAG |