| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198839 |
| Chromosome: | chromosome 9 |
| Location: | 3683031 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390801 | (1 of 1) PTHR11918//PTHR11918:SF77 - RADICAL SAM PROTEINS // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGCCAGGTAGAGCTGCGGGTGTAGGGT |
| Internal bar code: | TAGCGTATCGTCGGGTAAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 99.8533 |
| LEAP-Seq n confirming: | 6806 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGGCAATGTACCACTCCA |
| Suggested primer 2: | CGGTACCAGCCCAAACTAAA |