Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.198846 |
Chromosome: | chromosome 3 |
Location: | 3405033 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g166900 | CYP767A1,CYP15 | (1 of 1) 1.14.13.98 - Cholesterol 24-hydroxylase / Cytochrome P450 46A1; Cytochrome P450, CYP4 superfamily | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCGGCAGTGCTACATCTGCTCGTGCCT |
Internal bar code: | CTGCCAGGCGGTCGTCCGCTCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 306 |
LEAP-Seq percent confirming: | 89.3939 |
LEAP-Seq n confirming: | 118 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCAGTGTCATCGCCACCAT |
Suggested primer 2: | TGAGGTGGTGTGTGTGTGTG |