Insertion junction: LMJ.RY0402.198846_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.198846
Chromosome:chromosome 3
Location:3405033
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g166900 CYP767A1,CYP15 (1 of 1) 1.14.13.98 - Cholesterol 24-hydroxylase / Cytochrome P450 46A1; Cytochrome P450, CYP4 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTCGGCAGTGCTACATCTGCTCGTGCCT
Internal bar code:CTGCCAGGCGGTCGTCCGCTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:306
LEAP-Seq percent confirming:89.3939
LEAP-Seq n confirming:118
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAGTGTCATCGCCACCAT
Suggested primer 2:TGAGGTGGTGTGTGTGTGTG