| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.198848 |
| Chromosome: | chromosome 12 |
| Location: | 3307986 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497800 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTATGCACTCCAGCGGTATGGAGGCCAG |
| Internal bar code: | CACGTTTACTAGTGATTCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 718 |
| LEAP-Seq percent confirming: | 99.2291 |
| LEAP-Seq n confirming: | 2832 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGAACACCCGGACAGTT |
| Suggested primer 2: | GTCACTGCTGCTGCTACTGC |