Insertion junction: LMJ.RY0402.198849_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.198849
Chromosome:chromosome 12
Location:8878584
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g544662 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCTTGCAACCCCACCCCACCCCACGCAC
Internal bar code:TCATGTCTCGCGTCAGCGTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:856
LEAP-Seq percent confirming:95.4217
LEAP-Seq n confirming:13193
LEAP-Seq n nonconfirming:633
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:TGGACTAGGGTTGGCATTTC
Suggested primer 2:GGACCCGCTGTACTGTTCAT