| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.198901 |
| Chromosome: | chromosome 10 |
| Location: | 859289 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423800 | KU80 | (1 of 1) K10885 - ATP-dependent DNA helicase 2 subunit 2 (XRCC5, KU80, G22P2); ATP-dependent DNA helicase 2 subunit KU80 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCGGCAATGCTGGCGGCCGGCAAAGAG |
| Internal bar code: | GTAAGCACCCATACGCGGTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 498 |
| LEAP-Seq percent confirming: | 98.9894 |
| LEAP-Seq n confirming: | 2155 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAACAAAAGGACAGCCC |
| Suggested primer 2: | CTTGCTTCGTATGGCACTCA |