| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.198942 |
| Chromosome: | chromosome 12 |
| Location: | 1563863 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486700 | FAP271 | (1 of 7) PF07004 - Sperm-tail PG-rich repeat (SHIPPO-rpt); Flagellar Associated Protein 271 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGGCGCATGCCGCTTACGGTAGAGTAG |
| Internal bar code: | TTTGTGTCGGTTGCTGTAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 791 |
| LEAP-Seq percent confirming: | 99.5236 |
| LEAP-Seq n confirming: | 5432 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGTGCCGTAGCACGTGAA |
| Suggested primer 2: | AGGTCCGATGTTTTGTACGC |