| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.198966 |
| Chromosome: | chromosome 16 |
| Location: | 5016154 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684750 | (1 of 1) 1.1.1.184//1.1.1.307 - Carbonyl reductase (NADPH) / Xenobiotic ketone reductase // D-xylose reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGGCGGGCGTACGGATTGCGGCCAACCA |
| Internal bar code: | GGAGCCAGTGGGGTAGTAAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 99.8622 |
| LEAP-Seq n confirming: | 7249 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGTTGTCCCCTGCTTAC |
| Suggested primer 2: | CCGTGCGTTTGTATGTATGC |