| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198976 |
| Chromosome: | chromosome_4 |
| Location: | 2485192 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g221800 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCACAGGCGGCTTGCACGGACCCGTGGAT |
| Internal bar code: | CATCCCCCAGTGACGTGATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 160 |
| LEAP-Seq percent confirming: | 59.6244 |
| LEAP-Seq n confirming: | 127 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGCACTACTGTTTCGC |
| Suggested primer 2: | TCATCGTCATCCGAGTCAAA |