| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198977 |
| Chromosome: | chromosome 6 |
| Location: | 8850621 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g310650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCGCCGCCTCCGCGCTCTCCTTCACGT |
| Internal bar code: | AGGGGATGAACATGGATCTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 124 |
| LEAP-Seq percent confirming: | 45.4545 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTGCATTCAGTTGTCCA |
| Suggested primer 2: | ACGGTAACGCTTGATGTTCC |