| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.198991 |
| Chromosome: | chromosome 9 |
| Location: | 5576539 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400849 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTTCGAGCGTCGGCCGGTGACCCGGCT |
| Internal bar code: | GCAAGTCAGTTGGAAATGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 99.6426 |
| LEAP-Seq n confirming: | 3903 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGCGTTGCATCACAAAA |
| Suggested primer 2: | GCACGTCAAAAACGTCTCCT |