| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.199000 |
| Chromosome: | chromosome 3 |
| Location: | 5263856 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183300 | (1 of 4) 2.7.9.5 - Phosphoglucan, water dikinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTGGTGCAGAAGAGCAAGGGCAGGTGA |
| Internal bar code: | TATTTAGTAGTATAACGGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 891 |
| LEAP-Seq percent confirming: | 99.678 |
| LEAP-Seq n confirming: | 5881 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTATGGAAAACTGCCCG |
| Suggested primer 2: | TAACGACGCGTTGAAAGTTG |