| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199010 |
| Chromosome: | chromosome_1 |
| Location: | 264366 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g001657 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCACGCGCGTACGACTGTAAGCCACTGCCA |
| Internal bar code: | TGGCGGTCTGGCGCATCGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 96.0684 |
| LEAP-Seq n confirming: | 5840 |
| LEAP-Seq n nonconfirming: | 239 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGATGCTTTGTCATGGTG |
| Suggested primer 2: | GCGTGATGGGTACCTGAAGT |