| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199031 |
| Chromosome: | chromosome 14 |
| Location: | 1696462 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619400 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACCAGCGACGTCTCCTCCAACCGGTTCA |
| Internal bar code: | TCTGACCCCGCGATCACCCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 324 |
| LEAP-Seq percent confirming: | 98.445 |
| LEAP-Seq n confirming: | 3482 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCGATTTCGTACCTGGA |
| Suggested primer 2: | GGGAGCTGTACCTGCTCAAG |