| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199046 |
| Chromosome: | chromosome 10 |
| Location: | 1045069 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425200 | (1 of 1) 1.8.3.5 - Prenylcysteine oxidase / Prenylcysteine lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTGGCGAGTTGATGGTGGCAGTTCGTT |
| Internal bar code: | CTGCGGCCCTCCGGAAGAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1075 |
| LEAP-Seq percent confirming: | 97.1471 |
| LEAP-Seq n confirming: | 6538 |
| LEAP-Seq n nonconfirming: | 192 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTGTTCTCCGACTCTG |
| Suggested primer 2: | ATACGTTGTGCTGGGGAAAC |