| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.199075 |
| Chromosome: | chromosome_2 |
| Location: | 3326644 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g095090 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CATACATATTATGCTGTCCTAAGGCTGTTT |
| Internal bar code: | TATTACGTGTTGGACCAGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 442 |
| LEAP-Seq percent confirming: | 99.7275 |
| LEAP-Seq n confirming: | 5490 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACAAAGCAAGCCAGCGT |
| Suggested primer 2: | CGTGCAATTCCACACATACC |