| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.199136 |
| Chromosome: | chromosome 5 |
| Location: | 2846141 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237750 | (1 of 1) PF03980 - Nnf1 (Nnf1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTACGGTAGTGAGCGTACGGCGGTCCGC |
| Internal bar code: | GACCTAAGGCAACGTTTACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 941 |
| LEAP-Seq percent confirming: | 99.5989 |
| LEAP-Seq n confirming: | 745 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCACCTGCTTTACGTCT |
| Suggested primer 2: | CTTCCTGACCTTTCGTGAGC |