| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199139 |
| Chromosome: | chromosome 3 |
| Location: | 2725611 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g161578 | (1 of 6) IPR015893 - Tetracycline transcriptional regulator, TetR-like, C-terminal | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACGACCATCAGCAGTGAGGACCTGCTG |
| Internal bar code: | GTCCGGAAAGTAGCCAGCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 99.663 |
| LEAP-Seq n confirming: | 2070 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCGAATCAAGGTGACCAG |
| Suggested primer 2: | CCCTTTGTATGTGGGGTTTG |