Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.199182 |
Chromosome: | chromosome_6 |
Location: | 1918037 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g263450 | EEF1A3 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGTTTCCCGAATCCGCTCAGGGCACCCAA |
Internal bar code: | AGTAGGCAACCGACGTCCTCAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 225 |
LEAP-Seq percent confirming: | 98.9565 |
LEAP-Seq n confirming: | 1707 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTCCGTCACCCACAAGTAA |
Suggested primer 2: | CACCTACAGCGCCATGTCTA |