| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.199199 |
| Chromosome: | chromosome 3 |
| Location: | 4301927 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174700 | (1 of 1) 3.1.22.1 - Deoxyribonuclease II / Lysosomal DNase II | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCGGCGGGGAAGGCGATAGTGGTACCG |
| Internal bar code: | TCACGTTTTAAATAGTTCCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 328 |
| LEAP-Seq percent confirming: | 99.7403 |
| LEAP-Seq n confirming: | 768 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGAATCAGGGATGAAGG |
| Suggested primer 2: | TATCACTTCGATGGCAACCA |