| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.199206 |
| Chromosome: | chromosome 1 |
| Location: | 5241717 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036550 | ERV14 | (1 of 1) PTHR12290:SF11 - PROTEIN CORNICHON; Cornichon-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAACACGTGCTGCAAAGCTCCATTAAGG |
| Internal bar code: | CCTGGTGCCGCACGTAAGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 98.6551 |
| LEAP-Seq n confirming: | 3521 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTGGTACCGACACTTCC |
| Suggested primer 2: | ATCGCAGCTCATATCGCTTT |