| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.199225 |
| Chromosome: | chromosome 3 |
| Location: | 6668119 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGAAGCATGGCCATGCCGGCCGCGGCCA |
| Internal bar code: | TTTGCCCTACTTTACTTTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 867 |
| LEAP-Seq percent confirming: | 98.6726 |
| LEAP-Seq n confirming: | 446 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATACCCAGCGACCCTAC |
| Suggested primer 2: | GGAACAAGGGACAAGGTTGA |