| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.199231 |
| Chromosome: | chromosome 12 |
| Location: | 3532119 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513300 | CGL23 | Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCGGAGGAGGAGCGCGCCATAAAGCGG |
| Internal bar code: | ATGCGCCCCCCGTGGTGTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 97.4252 |
| LEAP-Seq n confirming: | 6281 |
| LEAP-Seq n nonconfirming: | 166 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCAGCACAACGGTATGAA |
| Suggested primer 2: | CCGTGTCGATCCTCAACTTT |