| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199240 |
| Chromosome: | chromosome 17 |
| Location: | 7144209 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g747647 | (1 of 2) 2.1.1.60 - Calmodulin-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTACCTCTAAACAGCTCCGGTCTCCGC |
| Internal bar code: | AGTTCCGGGCGAATAAAGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 85.1992 |
| LEAP-Seq n confirming: | 449 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAATGCTGGCCTCCTGTTC |
| Suggested primer 2: | AGCGGACTGGCTTTACAAGA |