| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.199250 |
| Chromosome: | chromosome 2 |
| Location: | 1714622 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g086100 | CGL40 | Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCATAGTGACGTTTGACGCACGGAATG |
| Internal bar code: | AAGAGTTTGTTCCGAGCAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 551 |
| LEAP-Seq percent confirming: | 99.7736 |
| LEAP-Seq n confirming: | 1763 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAGACGTCCAGTGATGA |
| Suggested primer 2: | ATACGACACAGGTTGAGCCC |