| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.199251 |
| Chromosome: | chromosome 6 |
| Location: | 2609909 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269650 | (1 of 3) 2.4.1.19 - Cyclomaltodextrin glucanotransferase / Cyclodextrin glycosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCGCAGCGTGAGAACCCTACTGTGGCAG |
| Internal bar code: | CGAGGTCATGTCAATTTTGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 145 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCAACCGCTCAAATTGTT |
| Suggested primer 2: | CCTCTGGACACATGCTCTCA |