| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.199271 |
| Chromosome: | chromosome 9 |
| Location: | 5563535 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400738 | (1 of 1) PTHR23012//PTHR23012:SF96 - MEMBRANE ASSOCIATED RING FINGER // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGAAACCATGGTGCATGCAGATGGCAGG |
| Internal bar code: | CCCGGTGGGATGGCACGAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 737 |
| LEAP-Seq percent confirming: | 99.7919 |
| LEAP-Seq n confirming: | 5755 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATGCCCACTCTACTTCC |
| Suggested primer 2: | AACGATAGGGTCGTGTCAGG |