| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.199277 |
| Chromosome: | chromosome 9 |
| Location: | 7501464 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g413950 | EFH6 | EF-hand Calcium binding protein; (1 of 5) PTHR23050:SF90 - CENTRIN-3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCACAGGCCCGACTCGCCAACCACCGC |
| Internal bar code: | GTCTGTCTTATAGTTCGATCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1045 |
| LEAP-Seq percent confirming: | 99.262 |
| LEAP-Seq n confirming: | 1076 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCAGGAGTCAAGTCGTC |
| Suggested primer 2: | ATCTATCAATGCAGGAGCCG |