| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.199279 |
| Chromosome: | chromosome 14 |
| Location: | 1680053 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619250 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGAATTCAAGGAACAATGGCGACGACT |
| Internal bar code: | GTTGGGGGCACCCGGGAAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1017 |
| LEAP-Seq percent confirming: | 99.4078 |
| LEAP-Seq n confirming: | 4532 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGCGTGTATGTAGGCT |
| Suggested primer 2: | TATCTCTTTCGCGCACACAC |