Insertion junction: LMJ.RY0402.199293_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.199293
Chromosome:chromosome_2
Location:3213090
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre02.g095072 FAP144 Flagellar Associated Protein sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GCCATTCAAAACGACGCGCGCGGCGGATGC
Internal bar code:GCTGCGGCCGGGCTCTGCTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:769
LEAP-Seq percent confirming:99.0099
LEAP-Seq n confirming:100
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAATCGTGGTATGTTCACGC
Suggested primer 2:TCATCAGAGACAACGGCAAG