| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.199293 |
| Chromosome: | chromosome 2 |
| Location: | 3213090 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095072 | FAP144 | (1 of 1) PF14886 - FAM183A and FAM183B related (FAM183); Flagellar Associated Protein 144 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATTCAAAACGACGCGCGCGGCGGATGC |
| Internal bar code: | GCTGCGGCCGGGCTCTGCTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 769 |
| LEAP-Seq percent confirming: | 99.0099 |
| LEAP-Seq n confirming: | 100 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATCGTGGTATGTTCACGC |
| Suggested primer 2: | TCATCAGAGACAACGGCAAG |