| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.199298 |
| Chromosome: | chromosome 16 |
| Location: | 5895710 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677250 | TEH5 | (1 of 6) 3.1.2.2 - Palmitoyl-CoA hydrolase / Long-chain fatty-acyl-CoA hydrolase; Thioesterase-like protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGGGAGGGCGCCAGTTTGCAGCGCTGG |
| Internal bar code: | ACGTCTCACACTGGTTGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 99.6593 |
| LEAP-Seq n confirming: | 585 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTTTAAGCGACGAAGCCA |
| Suggested primer 2: | ATGGGATGCGGTCACTAGAC |