| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199299 |
| Chromosome: | chromosome 16 |
| Location: | 3500332 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675602 | (1 of 1) K12669 - oligosaccharyltransferase complex subunit gamma (OST3, OST6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTGCTGGCAGCCAACGGTTCTCTCTGTC |
| Internal bar code: | GTCGACGACCGGCTCCCTTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 780 |
| LEAP-Seq percent confirming: | 98.1575 |
| LEAP-Seq n confirming: | 586 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATCCCGCAATTGAGATGT |
| Suggested primer 2: | TGTTATGGACCTGGGGGATA |