| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199302 |
| Chromosome: | chromosome_3 |
| Location: | 2528008 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g160000 | HDA3 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTATTGTATGACGCCGCGCGCGCGGTAGT |
| Internal bar code: | TCGTGGAATGTGTGGGGCACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 99.187 |
| LEAP-Seq n confirming: | 2562 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACAACCTTTCGGCCACC |
| Suggested primer 2: | GAGACACGTGCTGACGGTAA |