| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199342 |
| Chromosome: | chromosome 3 |
| Location: | 5122441 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGAGTGGCTTGATAGAGCATGAGACGC |
| Internal bar code: | CCGGGAGACGCTTGGTAGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 105 |
| LEAP-Seq percent confirming: | 96.988 |
| LEAP-Seq n confirming: | 161 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTGGGTCGGTCTAGTTC |
| Suggested primer 2: | CCAATACGAAGCTGCTGTCA |