| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.199355 |
| Chromosome: | chromosome 14 |
| Location: | 2394215 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g624400 | FAP99 | Flagellar Associated Protein 99 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATGCTGCTGCGGGCGCCCAGCTGCAGC |
| Internal bar code: | TTCTTTCCCTAGGTGCTCCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 801 |
| LEAP-Seq percent confirming: | 97.786 |
| LEAP-Seq n confirming: | 265 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGGCGCAGAGTAGATTG |
| Suggested primer 2: | TCACGACAGAGCCCTTTTCT |