| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.199369 |
| Chromosome: | chromosome 3 |
| Location: | 6153898 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192000 | SEC13L,SEH1 | COP-II coat subunit, nucleoporin; (1 of 1) K14299 - nucleoporin SEH1 (SEH1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGAGGTTTGCTTCGGACTAGGAGGAAA |
| Internal bar code: | ACTAACCGAGAAGAGGTGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 99.7275 |
| LEAP-Seq n confirming: | 2196 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCCCCTCACCTTGACTT |
| Suggested primer 2: | GGCGTTGTCCGTGTCTTTAT |