| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199419 |
| Chromosome: | chromosome 6 |
| Location: | 4243677 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278244 | (1 of 2) 2.4.2.7 - Adenine phosphoribosyltransferase / Transphosphoribosidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGAGGAGCAGGTGGGGGCCGAGGAGCAG |
| Internal bar code: | GGAGGGGACTAGTCGAGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 89.8658 |
| LEAP-Seq n confirming: | 2545 |
| LEAP-Seq n nonconfirming: | 287 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTTAGTTTGGGCTGGTA |
| Suggested primer 2: | TCGGAGTTGCAACAGATGAG |