| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.199434 |
| Chromosome: | chromosome 3 |
| Location: | 547846 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145387 | FAP239 | Coiled-Coil Flagellar Associated Protein 239 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCTGCTGAGGAGGGGCCATGCCTAGGAG |
| Internal bar code: | CGGCGTTTGACGGGCGGGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 688 |
| LEAP-Seq percent confirming: | 99.1701 |
| LEAP-Seq n confirming: | 478 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTGTCCCCTATCTCCCT |
| Suggested primer 2: | GCGATCATTTGCTGTCAAGA |