| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199444 |
| Chromosome: | chromosome 11 |
| Location: | 909332 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467657 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTGCGTCGTGGCCTTTGCGTGCCAAAG |
| Internal bar code: | GTAAGAGCGGAAGAGGGATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 298 |
| LEAP-Seq percent confirming: | 99.7724 |
| LEAP-Seq n confirming: | 2192 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGGTGCTAAAGTGTCCG |
| Suggested primer 2: | CTCTCAGGAAACAAGCCCAG |