| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.199455 |
| Chromosome: | chromosome_10 |
| Location: | 3172282 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g442200 | ERG6,ERG26 | 3-beta hydroxysteroid dehydrogenase/epimerase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGGGCGTGGCAGGGCTCTGAAGCAGCGAC |
| Internal bar code: | CGCCCTGAAAGCGGAGTAATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 493 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGATCATGTTCTCGGGCG |
| Suggested primer 2: | ACCAAGAACATTGTGGAGGC |