| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199469 |
| Chromosome: | chromosome 10 |
| Location: | 4582452 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452550 | HOPP1,HOP1 | Homologous-pairing protein 1; (1 of 3) PF02301 - HORMA domain (HORMA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCCTGTTCTGTATTGTGCCGTGCTGTT |
| Internal bar code: | AAGATGACCGCGTTCGCTCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 92.4138 |
| LEAP-Seq n confirming: | 536 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGCCACACAGCAGTCTAA |
| Suggested primer 2: | CGAGGAAGAGCAAGCATACC |