| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.199472 |
| Chromosome: | chromosome 13 |
| Location: | 4042125 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g591150 | HTA38,HTA22 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTAACGGGGTGCCGAATGCCGCGCATC |
| Internal bar code: | GCGTGGGGCTTGGGTTAGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 961 |
| LEAP-Seq percent confirming: | 99.7499 |
| LEAP-Seq n confirming: | 5185 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCTACCTAAAGAAGGGCA |
| Suggested primer 2: | TAGAGTCCGAGCGTGAGGTT |