| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.199484 |
| Chromosome: | chromosome_2 |
| Location: | 2808952 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g094450 | PHC21 | Cell wall protein pherophorin-C21 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGGCTGTATGTGGAGCTGAATGACTTTGA |
| Internal bar code: | CTATATACACGACTGCCGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 99.4531 |
| LEAP-Seq n confirming: | 2364 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACAAGAGCCAGGGAGCAC |
| Suggested primer 2: | AGGTTAAGGCGGGTAGCAAT |