Insertion junction: LMJ.RY0402.199484_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.199484
Chromosome:chromosome 2
Location:2809015
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g094450 PHC38,PHC21 Pherophorin-chlamydomonas homolog 38; (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTAGACCCAGCGAGGCGCACGTCCCCAT
Internal bar code:GCCACCGGAATTACATAAATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:169
LEAP-Seq percent confirming:93.8424
LEAP-Seq n confirming:381
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGGCTATGTGTACAGCCG
Suggested primer 2:AGGTTAAGGCGGGTAGCAAT