| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.199489 |
| Chromosome: | chromosome 3 |
| Location: | 4665645 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177550 | (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTGGTGCGTCGGAGCTCTGGTTCATAC |
| Internal bar code: | CAAGAAGAACACGAGGCCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 489 |
| LEAP-Seq percent confirming: | 98.9058 |
| LEAP-Seq n confirming: | 3254 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCTCCACGACCTGTGATT |
| Suggested primer 2: | TTTATGTTGGCTCCTCCGTC |