| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.199491 |
| Chromosome: | chromosome 3 |
| Location: | 6345742 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193700 | (1 of 1) PTHR10992//PTHR10992:SF749 - ALPHA/BETA HYDROLASE FOLD-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCGGCGGGTGAGGGAGCGGGGGCGAAC |
| Internal bar code: | CGCTTTCCTTACATTGCTCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 99.7782 |
| LEAP-Seq n confirming: | 2699 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGCGGAAAGTTGAAACGA |
| Suggested primer 2: | ATGGCTTATGGCATGGGTAG |